Description
UCLA HEALTH
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Description
Under the
direction of the Principal Investigator (PI), the Staff Research Associate III
(SRA III) is responsible for executing cellular and molecular techniques as
part of the basic, translational and clinical research. Duties include the cell
culture of primary cells and cell lines as well as other molecular biology
techniques including but not limited to cloning, mutagenesis, sequencing,
polymerase chain reaction (PCR), Western Blot, immunohistochemistry/immunocytochemistry,
and cryosectioning. The SRA III will perform imaging acquisition and imaging
and data analysis, keep good documentation records in compliance, assist with
pre-clinical experiments and actively participate in qualifications and
clinical manufacturing part of a Phase I clinical trial.
The SRA III
will also be in charge of lab management and lab maintenance including but not
limited to overseeing lab activities ensuring that a safe, clean, organized,
well equipped/stocked and compliant environment is maintained.
Qualifications
All
Required:
- Bachelor’s
degree in life science (not including psychology) and or equivalent experience
and/or training -
Knowledge of
molecular biology techniques -
Skills and
extensive experience in human and mouse cell culture technique -
Knowledge of
cell culture, including thawing cells, culturing cells and freezing cells -
Skills in
cryosection -
Skills in
isolating and purifying high quality DNA and RNA -
Skill in
subcloning of cell lines -
Ability to use
molecular biology software -
Ability and
experience to perform immunohistochemistry -
Ability to
perform protein analysis by gel electrophoresis and Western Blots -
Experience with
tissue and cell imaging and analysis -
Ability to
establish and maintain records and supply stocks -
Skill in
maintaining the lab and equipment in working condition -
Ability to
establish and maintain cooperative working relationships with co-workers -
Demonstrated
experience using general lab equipment such as pipettes, centrifuges,
balances, incubators, thermal cyclers, dry blocks, shakers, autoclaves,
water baths, liquid nitrogen tanks, DNA synthesizer, pH meter, etc. -
Ability to work
with mice, setting up breeding pairs and recording births and weaning animals -
Ability to
plan, execute and coordinate all the GMP/GLP activities related to the
pre-clinical and Phase I clinical projects in collaboration with the
research teams -
Knowledge of
GLP and GMP -
Ability to
manage and maintain the lab ensuring that a safe, clean and compliant
environment is maintained -
Ability to work
on multiple projects, often changing from one task to another -
Ability to
establish and maintain cooperative working relationships with personnel both in
and outside of the laboratory group -
Communicate
clearly, concisely and accurately in verbal and written formats -
Ability to
summarize and present data